Anti-Human MUC-1 (Tn/STn) Recombinant Antibody (5E5#) (HB102107)

Name: Anti-Human MUC-1 (Tn/STn) Recombinant Antibody (5E5#)
Brand: abinScience
SKU: HB102107
Price: 191 USD
Availability: InStock

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Overview

Catalog No.	HB102107
Species reactivity	Human
Applications	ELISA, FCM, IHC, WB
Host species	Human
Clonality	Monoclonal
Isotype	IgG1
Clone ID	5E5#
Tested applications	FCM
Target	Breast carcinoma-associated antigen DF3, CA 15-3, CD227, Cancer antigen 15-3, Carcinoma-associated mucin, EMA, Episialin, H23AG, KL-6, Krebs von den Lungen-6, MUC-1, MUC1, MUC1-CT, MUC1-NT, MUC1-alpha, MUC1-beta, Mucin-1, Mucin-1 subunit alpha, Mucin-1 subunit beta, PEM, PEMT, PUM, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial membrane antigen, Tumor-associated mucin
Endotoxin level	< 10 EU/mg
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P15941
Form	Liquid
Storage buffer	0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	Mucin-1 (CD227/MUC1) is a ~122 kDa protein. The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.
Note	For research use only.

Images

Bioactivity

SEC-HPLC detection for Anti-Human MUC1-Tn/STn Antibody (5E5#).
SDS-PAGE

SDS-PAGE for Anti-Human MUC1-Tn/STn Antibody (5E5#)
Flow CytoMetry

Flow-cytometry using anti-human CD227 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD227 monoclonal antibody (Catalog: HB102107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.