Anti-Human MUSK Polyclonal Antibody (HT224014)

Name: Anti-Human MUSK Polyclonal Antibody
Brand: abinScience
SKU: HT224014
Price: 96 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HT224014

Description

Anti-Human MUSK Polyclonal Antibody (HT224014) is a rabbit polyclonal antibody detecting MuSK in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MUSK (Leu24-Glu305).

Target

Muscle, skeletal receptor tyrosine-protein kinase, 2.7.10.1, Muscle-specific tyrosine-protein kinase receptor, MuSK, Muscle-specific kinase receptor, MUSK

Purification

Purified by antigen affinity column.

Accession

O15146

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Muscle, skeletal receptor tyrosine-protein kinase (MUSK) is a ~97 kDa protein. Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering.

1. Tan-Sindhunata, MB. et al. (2015) European journal of human genetics : EJHG 23, 1151-7. PMID: 25537362

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human MUSK polyclonal antibody at 1ug/mL on recombinant Human MUSK (Catalog No. HT224012).

References

Datasheet