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Anti-Human NASP Polyclonal Antibody (HC209014)

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Anti-Human NASP Polyclonal Antibody
Anti-Human NASP Polyclonal Antibody
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Overview
Catalog No.HC209014
Description
Anti-Human NASP Polyclonal Antibody (HC209014) is a rabbit polyclonal antibody detecting NASP. Suitable for Mouse, Human, and Rat.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human NASP (Gly513-Gly681).
Target NASP, Nuclear autoantigenic sperm protein
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P49321
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Nuclear autoantigenic sperm protein (NASP) is a ~85 kDa protein. Component of the histone chaperone network. Binds and stabilizes histone H3-H4 not bound to chromatin to maintain a soluble reservoir and modulate degradation by chaperone-mediated autophagy. Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.

1. Cook, AJ. et al. (2011) Molecular cell 44, 918-27. PMID: 22195965
Note For research use only
Images
  • Anti-Human NASP Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-NASP polyclonal antibody at 1ug/mL on various samples.
    Lane 1: K562 cell lysate
    Lane 2: 293T cell lysate
    Lane 3: HL-60 cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human NASP Polyclonal Antibody

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