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Anti-Human NCAPH Polyclonal Antibody (HC485014)

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Overview
Catalog No.HC485014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human NCAPH (Glu471-Pro526).
Target CAPH, BRRN1, hCAP-H, Barren homolog protein 1, XCAP-H homolog, Non-SMC condensin I complex subunit H, BRRN, Chromosome-associated protein H, NCAPH, Condensin complex subunit 2, KIAA0074
Purification Purified by antigen affinity column.
Accession Q15003
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Condensin complex subunit 2 (NCAPH) is a ~82 kDa protein. Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size.

1. Kimura, K. et al. (2001) The Journal of biological chemistry 276, 5417-20. PMID: 11136719
2. Martin, CA. et al. (2016) Genes & development 30, 2158-2172. PMID: 27737959
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Human NCAPH Polyclonal Antibody

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