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Anti-Human NDEL1 Polyclonal Antibody (HP160014)

Anti-Human NDEL1 Polyclonal Antibody
Anti-Human NDEL1 Polyclonal Antibody
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Overview
Catalog No.HP160014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human NDEL1 (Asp8-Lys193).
Target Protein Nudel, EOPA, Nuclear distribution protein nudE-like 1, NUDEL, MITAP1, NDEL1, Mitosin-associated protein 1
Purification Purified by antigen affinity column.
Accession Q9GZM8
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Nuclear distribution protein nudE-like 1 (NDEL1) is a ~38 kDa protein. Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome.

1. Zhang, Y. et al. (2022) Structure (London, England : 1993) 30, 386-395.e5. PMID: 34793709
Note For research use only.
Images
  • Anti-Human NDEL1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-NDEL1 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Mouse brain lysate
    Lane 2: Rat brain lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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