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Anti-Human NDUFA13 Polyclonal Antibody (HP376014)

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Overview
Catalog No.HP376014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human NDUFA13 (Met51-Thr144).
Target Gene associated with retinoic and IFN-induced mortality 19 protein, Gene associated with retinoic and interferon-induced mortality 19 protein, CI-B16.6, Cell death regulatory protein GRIM-19, NADH-ubiquinone oxidoreductase B16.6 subunit, GRIM-19, Complex I-B16.6, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13, GRIM19, NDUFA13
Purification Purified by antigen affinity column.
Accession Q9P0J0
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (NDUFA13) is a ~16 kDa protein. Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Involved in the interferon/all-trans-retinoic acid (IFN/RA) induced cell death. This apoptotic activity is inhibited by interaction with viral IRF1.

1. Stroud, DA. et al. (2016) Nature 538, 123-126. PMID: 27626371
2. Barnich, N. et al. (2005) The Journal of biological chemistry 280, 19021-6. PMID: 15753091
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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