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Anti-Human NFKBIZ Polyclonal Antibody (HP342014)

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Overview
Catalog No.HP342014
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human NFKBIZ (Gln454-Leu586).
Target NF-kappa-B inhibitor zeta, MAIL, IkB-zeta, IL-1 inducible nuclear ankyrin-repeat protein, INAP, NFKBIZ, I-kappa-B-zeta, IKBZ, Molecule possessing ankyrin repeats induced by lipopolysaccharide, IkappaBzeta
Purification Purified by antigen affinity column.
Accession Q9BYH8
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

NF-kappa-B inhibitor zeta (NFKBIZ) is a ~78 kDa protein. Involved in regulation of NF-kappa-B transcription factor complexes. Inhibits NF-kappa-B activity without affecting its nuclear translocation upon stimulation. Inhibits DNA-binding of RELA and NFKB1/p50, and of the NF-kappa-B p65-p50 heterodimer and the NF-kappa-B p50-p50 homodimer. Also seems to activate NF-kappa-B-mediated transcription. In vitro, upon association with NFKB1/p50 has transcriptional activation activity and, together with NFKB1/p50 and RELA, is recruited to LCN2 promoters.

1. Totzke, G. et al. (2006) The Journal of biological chemistry 281, 12645-54. PMID: 16513645
2. Cowland, JB. et al. (2006) Journal of immunology (Baltimore, Md. : 1950) 176, 5559-66. PMID: 16622025
Note For research use only.
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Anti-Human NFKBIZ Polyclonal Antibody

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