Anti-Human NINJ1 Polyclonal Antibody (HA042014)

Name: Anti-Human NINJ1 Polyclonal Antibody
Brand: abinScience
SKU: HA042014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HA042014

Description

Anti-Human NINJ1 Polyclonal Antibody (HA042014) is a rabbit polyclonal antibody detecting NINJ1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NINJ1 (Asp2-Leu80 & Gln142-Gln152).

Target

Ninjurin-1, Nerve injury-induced protein 1, Secreted ninjurin-1, Soluble ninjurin-1, NINJ1

Purification

Purified by antigen affinity column.

Accession

Q92982

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ninjurin-1 (NINJ1) is a ~16 kDa protein. Effector of various programmed cell death, such as pyroptosis and necroptosis, which mediates plasma membrane rupture (cytolysis). Oligomerizes in response to death stimuli and forms ring-like structures on the plasma membrane: acts by cutting and shedding membrane disks, like a cookie cutter, leading to membrane damage and loss that cannot be repaired by the cell. Plasma membrane rupture leads to release intracellular molecules named damage-associated molecular patterns (DAMPs) that propagate the inflammatory response. Mechanistically, mediates plasma membrane rupture by introducing hydrophilic faces of 2 alpha helices into the hydrophobic membrane. Induces plasma membrane rupture downstream of Gasdermin (GSDMA, GSDMB, GSDMC, GSDMD, or GSDME) or MLKL during pyroptosis or necroptosis, respectively.

1. Kayagaki, N. et al. (2021) Nature 591, 131-136. PMID: 33472215

2. Borges, JP. et al. (2022) eLife 11. PMID: 36468682

3. Kayagaki, N. et al. (2023) Nature 618, 1072-1077. PMID: 37196676

4. Degen, M. et al. (2023) Nature 618, 1065-1071. PMID: 37198476

5. David, L. et al. (2024) Cell 187, 2224-2235.e16. PMID: 38614101

6. Sahoo, B. et al. (2025) Cell 188, 292-302.e11. PMID: 39667936

Note

For research use only.

Images

References

Datasheet

Anti-Human NINJ1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human NINJ1 Polyclonal Antibody (HA042014)

Overview

Images

References

Datasheet

SDS

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