Anti-Human NME1 Polyclonal Antibody (HB870014)

Name: Anti-Human NME1 Polyclonal Antibody
Brand: abinScience
SKU: HB870014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB870014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NME1 (Met1-Glu152).

Target

NDPKA, NME1, NDP kinase A, Nucleoside diphosphate kinase A, Granzyme A-activated DNase, NM23, Metastasis inhibition factor nm23, GAAD, Tumor metastatic process-associated protein, NDK A, NM23-H1

Purification

Purified by antigen affinity column.

Accession

P15531

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Nucleoside diphosphate kinase A (NME1) is a ~17 kDa protein. Catalyzes the transfer of a gamma-phosphoryl group from a nucleoside triphosphate, mainly ATP, to a nucleoside diphosphate via a ping-pong mechanism involving a phosphohistidine intermediate, therefore contributing to the nucleoside triphosphate homeostasis. Also phosphorylates geranyl pyrophosphate (GPP) and farnesyl pyrophosphate (FPP), linking it to isoprenoid metabolism. Additionally, functions as a non-specific serine/threonine kinase and histidine protein kinase, transferring phosphoryl groups from its active site to target proteins. May function as a Mg(2+)-dependent single-stranded DNA endonuclease as part of the SET complex, cooperating with the 3'-5' exonuclease TREX1 to mediate apoptotic DNA fragmentation in cytotoxic T lymphocytes. Reported to nick one DNA strand, enabling TREX1 to remove nucleotides from the free 3' end, enhancing DNA damage and suppressing DNA end reannealing and repair.

1. Wagner, PD. et al. (2000) The Journal of biological chemistry 275, 35570-6. PMID: 10952986

2. Ma, D. et al. (2004) The Journal of biological chemistry 279, 18073-84. PMID: 14960567

3. Yoon, JH. et al. (2005) Biochemistry 44, 15774-86. PMID: 16313181

4. Gilles, AM. et al. (1991) The Journal of biological chemistry 266, 8784-9. PMID: 1851158

5. Kim, MS. et al. (2013) Acta crystallographica. Section D, Biological crystallography 69, 669-80. PMID: 23519676

6. Yu, BYK. et al. (2021) Redox biology 44, 101978. PMID: 33903070

7. MacDonald, NJ. et al. (1996) The Journal of biological chemistry 271, 25107-16. PMID: 8810265

8. Freije, JM. et al. (1997) The Journal of biological chemistry 272, 5525-32. PMID: 9038158

9. Engel, M. et al. (1995) European journal of biochemistry 234, 200-7. PMID: 8529641

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-NME1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse brain lysate
Lane 2: Mouse lung lysate
Lane 3: Mouse liver lysate
Lane 4: Rat brain lysate
Lane 5: Rat lung lysate
Lane 6: Rat liver lysate

References

Datasheet

Anti-Human NME1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-Human NME1 Polyclonal Antibody (HB870014)

Overview

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References

Datasheet

SDS

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