Anti-Human Notch-1 Recombinant Antibody (SAA0131) (HW570107)

Name: Anti-Human Notch-1 Recombinant Antibody (SAA0131)
Brand: abinScience
SKU: HW570107
Price: 191 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.	HW570107
Species reactivity	Human
Applications	FCM
Host species	Human
Clonality	Monoclonal
Isotype	IgG2, lambda
Clone ID	SAA0131
Tested applications	FCM
Target	hN1, TAN1, NEXT, NICD, Notch 1, Neurogenic locus notch homolog protein 1, Translocation-associated notch protein TAN-1, NOTCH1
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P46531
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Neurogenic locus notch homolog protein 1 (NOTCH1) is a ~272 kDa protein. Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus.
References	1. NOTCH1 notch receptor 1 [Homo sapiens (human)] Gene ID: 4851, updated on 28-Oct-2019. [HW570107] 2. toyanova T, et al. Activation of Notch1 synergizes with multiple pathways in promoting castration-resistant prostate cancer. Proc Natl Acad Sci U S A. 2016 Oct 18;113(42):E6457-E6466. [HW570107]
Note	For research use only.

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Flow Cytometry

Flow-cytometry using anti-human NOTCH1 antibody. Human MOLT-4 cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human NOTCH1 antibody monoclonal antibody (Catalog # HW570107 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet