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Anti-Human NOX5 Polyclonal Antibody (HV641014)

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Overview
Catalog No.HV641014
Description
Anti-Human NOX5 Polyclonal Antibody (HV641014) is a rabbit polyclonal antibody detecting NOX5 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human NOX5 (Tyr605-Phe765).
Target NADPH oxidase 5, 1.6.3.-, NOX5
Purification Purified by antigen affinity column.
Accession Q96PH1
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

NADPH oxidase 5 (NOX5) is a ~86 kDa protein. Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. Also synthesizes NAADP from its reduced NAADPH form which promotes Ca(2+) signaling during T cell activation. May play a role in cell growth and apoptosis.

1. Brar, SS. et al. (2003) American journal of physiology. Cell physiology 285, C353-69. PMID: 12686516
2. Gu, F. et al. (2021) Science signaling 14, eabe3800. PMID: 34784249
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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