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Anti-Human NR0B2 Polyclonal Antibody (HS713014)

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Anti-Human NR0B2 Polyclonal Antibody
Anti-Human NR0B2 Polyclonal Antibody
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Overview
Catalog No.HS713014
Description
Anti-Human NR0B2 Polyclonal Antibody (HS713014) is a rabbit polyclonal antibody detecting NR0B2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human NR0B2 (Met1-Arg257).
Target NR0B2
Purification Purified by antigen affinity column.
Accession Q15466
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesNuclear receptor subfamily 0 group B member 2, Orphan nuclear receptor SHP, Small heterodimer partner, NR0B2, SHP
Background

Nuclear receptor subfamily 0 group B member 2 (NR0B2) is a ~28 kDa protein. Transcriptional regulator that acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with which it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated transcription complex for NEUROD1. Essential component of the liver circadian clock which via its interaction with NR1D1 and RORG regulates NPAS2-mediated hepatic lipid metabolism. Regulates the circadian expression of cytochrome P450 (CYP) enzymes.

1. Musille, PM. et al. (2012) Nature structural & molecular biology 19, 532-S2. PMID: 22504882
2. Kim, JY. et al. (2004) Molecular endocrinology (Baltimore, Md.) 18, 776-90. PMID: 14752053
Note For research use only.
Images
  • Anti-Human NR0B2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-NR0B2 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse liver lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human NR0B2 Polyclonal Antibody

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