Anti-Human NR1D1 Polyclonal Antibody (HB802014)

Name: Anti-Human NR1D1 Polyclonal Antibody
Brand: abinScience
SKU: HB802014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB802014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NR1D1 (Thr431-Gln614).

Target

THRAL, EAR1, EAR-1, NR1D1, Rev-erbA-alpha, Nuclear receptor subfamily 1 group D member 1, HREV, V-erbA-related protein 1

Purification

Purified by antigen affinity column.

Accession

P20393

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Nuclear receptor subfamily 1 group D member 1 (NR1D1) is a ~66 kDa protein. Transcriptional repressor which coordinates circadian rhythm and metabolic pathways in a heme-dependent manner. Integral component of the complex transcription machinery that governs circadian rhythmicity and forms a critical negative limb of the circadian clock by directly repressing the expression of core clock components BMAL1, CLOCK and CRY1. Also regulates genes involved in metabolic functions, including lipid and bile acid metabolism, adipogenesis, gluconeogenesis and the macrophage inflammatory response. Acts as a receptor for heme which stimulates its interaction with the NCOR1/HDAC3 corepressor complex, enhancing transcriptional repression. Recognizes two classes of DNA response elements within the promoter of its target genes and can bind to DNA as either monomers or homodimers, depending on the nature of the response element.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-NR1D1 polyclonal antibody at 1µg/ml on various samples.
Lane 1: Mouse skeletal muscle lysate
Lane 2: Rat lung lysate
Lane 3: Rat liver lysate
Lane 4: Rat skeletal muscle lysate

References

Datasheet

Anti-Human NR1D1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human NR1D1 Polyclonal Antibody (HB802014)

Overview

Images

References

Datasheet

SDS

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