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Anti-Human NRCAM Polyclonal Antibody (HP177014)

Anti-Human NRCAM Polyclonal Antibody
Anti-Human NRCAM Polyclonal Antibody
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Overview
Catalog No.HP177014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human NRCAM (Thr47-Ala451).
Target Ng-CAM-related, KIAA0343, hBravo, NRCAM, NgCAM-related cell adhesion molecule, Neuronal cell adhesion molecule, Neuronal surface protein Bravo, Nr-CAM
Purification Purified by antigen affinity column.
Accession Q92823
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Neuronal cell adhesion molecule (NRCAM) is a ~143 kDa protein. Cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding. Plays a role in mediating cell-cell contacts between Schwann cells and axons. Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons.

Note For research use only.
Images
  • Anti-Human NRCAM Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-NRCAM polyclonal antibody at 1μg/ml on various samples.
    Lane 1: Mouse brain lysate
    Lane 2: Mouse cerebellum lysate
    Lane 3: Rat brain lysate
    Lane 4: Rat cerebellum lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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