Anti-Human NSFL1C Polyclonal Antibody (HP366014)

Name: Anti-Human NSFL1C Polyclonal Antibody
Brand: abinScience
SKU: HP366014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HP366014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NSFL1C (Ser271-Thr370).

Target

NSFL1 cofactor p47, p97 cofactor p47, NSFL1C, UBXN2C, UBX domain-containing protein 2C

Purification

Purified by antigen affinity column.

Accession

Q9UNZ2

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

NSFL1 cofactor p47 (NSFL1C) is a ~40 kDa protein. Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Inhibits the activity of CTSL (in vitro). Together with UBXN2B/p37, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase.

1. Soukenik, M. et al. (2004) FEBS letters 576, 358-62. PMID: 15498563

2. Kress, E. et al. (2013) The Journal of cell biology 201, 559-75. PMID: 23649807

Note

For research use only.

Images

References

Datasheet

Anti-Human NSFL1C Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human NSFL1C Polyclonal Antibody (HP366014)

Overview

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References

Datasheet

SDS

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