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Anti-Human OLIG2 Polyclonal Antibody (HC300014)

Anti-Human OLIG2 Polyclonal Antibody
Anti-Human OLIG2 Polyclonal Antibody
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Overview
Catalog No.HC300014
Description
Anti-Human OLIG2 Polyclonal Antibody (HC300014) is a rabbit polyclonal antibody detecting OLIG2 in ELISA, IHC, WB. Suitable for Human, Mouse, and Chicken.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human OLIG2 (Thr102-His180).
Target Protein kinase C-binding protein 2, bHLHe19, bHLHb1, PRKCBP2, Class E basic helix-loop-helix protein 19, Protein kinase C-binding protein RACK17, Oligodendrocyte transcription factor 2, BHLHE19, Oligo2, RACK17, BHLHB1, Class B basic helix-loop-helix protein 1, OLIG2
Purification Purified by antigen affinity column.
Accession Q13516
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Oligodendrocyte transcription factor 2 (OLIG2) is a ~32 kDa protein. Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Functions together with ZNF488 to promote oligodendrocyte differentiation. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.

Note For research use only.
Images
  • Anti-Human OLIG2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-OLIG2 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: N2A cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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