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Anti-Human P3H4 Polyclonal Antibody (HP878014)

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Overview
Catalog No.HP878014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human P3H4 (Arg133-Gln378).
Target NOL55, Prolyl 3-hydroxylase family member 4, SC65, Leprecan-like protein 4, Synaptonemal complex protein SC65, P3H4, LEPREL4, Nucleolar autoantigen No55, Endoplasmic reticulum protein SC65
Purification Purified by antigen affinity column.
Accession Q92791
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Endoplasmic reticulum protein SC65 (P3H4) is a ~50 kDa protein. Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linking of collagen fibrils. Required for normal bone density and normal skin stability via its role in hydroxylation of lysine residues in collagen alpha chains and in collagen fibril assembly.

Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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Anti-Human P3H4 Polyclonal Antibody

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