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Anti-Human PADI6 Polyclonal Antibody (HB365014)

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Overview
Catalog No.HB365014
Description
Anti-Human PADI6 Polyclonal Antibody (HB365014) is a rabbit polyclonal antibody detecting Protein-arginine deiminase type-6; 3.5.3.15; Peptidyl arginine deiminase-like protein; Peptidylarginine deiminase VI; hPADVI; Protein-arginine deiminase type VI; PADI6; PAD6 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human PADI6 (Val2-Pro694).
Target Protein-arginine deiminase type-6; 3.5.3.15; Peptidyl arginine deiminase-like protein; Peptidylarginine deiminase VI; hPADVI; Protein-arginine deiminase type VI; PADI6; PAD6
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q6TGC4
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Inactive protein-arginine deiminase type-6 (PADI6) is a ~77 kDa protein. Structural constituent of cytoplasmic lattices, which plays a key role in early embryonic development. Cytoplasmic lattices consist in fibrous structures found in the cytoplasm of oocytes and preimplantation embryos. They are required to store maternal proteins critical for embryonic development, such as ribosomal proteins and proteins that control epigenetic reprogramming of the preimplantation embryo, and prevent their degradation or activation. In contrast to other members of the family, does not show protein-arginine deiminase activity due to its inability to bind Ca(2+).

1. Jentoft, IMA. et al. (2023) Cell 186, 5308-5327.e25. PMID: 37922900
2. Ranaivoson, FM. et al. (2024) IUCrJ 11, 395-404. PMID: 38656308
3. Williams, JPC. et al. (2024) Computational and structural biotechnology journal 23, 3258-3269. PMID: 39286527
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human PADI6 Polyclonal Antibody

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