Anti-Human PDPN/Podoplanin Polyclonal Antibody (HV106014)

Name: Anti-Human PDPN/Podoplanin Polyclonal Antibody
Brand: abinScience
SKU: HV106014
Price: 168 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HV106014

Description

Anti-Human PDPN/Podoplanin Polyclonal Antibody (HV106014) is a rabbit polyclonal antibody detecting Podoplanin in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PDPN/Podoplanin (Ala23-Leu131).

Target

Podoplanin, Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A, 29kDa cytosolic podoplanin intracellular domain, PICD, PDPN, GP36, PSEC0003, PSEC0025

Purification

Purified by antigen affinity column.

Accession

Q86YL7

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Podoplanin (PDPN) is a ~16 kDa protein. Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation. Interaction with CD9, on the contrary, attenuates platelet aggregation induced by PDPN. Through MSN or EZR interaction promotes epithelial-mesenchymal transition (EMT) leading to ERZ phosphorylation and triggering RHOA activation leading to cell migration increase and invasiveness. Interaction with CD44 promotes directional cell migration in epithelial and tumor cells.

1. Kato, Y. et al. (2003) The Journal of biological chemistry 278, 51599-605. PMID: 14522983

2. Kaneko, M. et al. (2004) The Journal of biological chemistry 279, 38838-43. PMID: 15231832

3. Kaneko, MK. et al. (2007) FEBS letters 581, 331-6. PMID: 17222411

4. Suzuki-Inoue, K. et al. (2007) The Journal of biological chemistry 282, 25993-6001. PMID: 17616532

5. Christou, CM. et al. (2008) The Biochemical journal 411, 133-40. PMID: 18215137

6. Nakazawa, Y. et al. (2008) Blood 112, 1730-9. PMID: 18541721

7. Martín-Villar, E. et al. (2006) Journal of cell science 119, 4541-53. PMID: 17046996

8. Fernández-Muñoz, B. et al. (2011) The international journal of biochemistry & cell biology 43, 886-96. PMID: 21376833

9. Martín-Villar, E. et al. (2010) Molecular biology of the cell 21, 4387-99. PMID: 20962267

10. Cueni, LN. et al. (2009) Experimental cell research 315, 1715-23. PMID: 19268462

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human PDPN/Podoplanin polyclonal antibody at 1ug/mL on recombinant Human PDPN/Podoplanin (Catalog No. HV106022).

References

Datasheet