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Anti-Human PEBP1 Polyclonal Antibody (HB091014)

Anti-Human PEBP1 Polyclonal Antibody
Anti-Human PEBP1 Polyclonal Antibody
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Overview
Catalog No.HB091014
Description
Anti-Human PEBP1 Polyclonal Antibody (HB091014) is a rabbit polyclonal antibody detecting PEBP1 in ELISA, IHC, WB. Suitable for Macaca fascicularis, Mus musculus, Human, Pongo abelii, and Bovine.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
  • Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PEBP1 (Met1-Lys187).
Target PEBP1
Purification Purified by antigen affinity column.
Accession P30086
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesPhosphatidylethanolamine-binding protein 1, PEBP-1, HCNPpp, Neuropolypeptide h3, Prostatic-binding protein, Raf kinase inhibitor protein, RKIP, Hippocampal cholinergic neurostimulating peptide, HCNP, PEBP1, PBP, PEBP
Background

Phosphatidylethanolamine-binding protein 1 (PEBP1) is a ~21 kDa protein. Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase. Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation.

Note For research use only.
Images
  • Anti-Human PEBP1 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PEBP1 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: N2A cell lysate
    Lane 2: PC-12 cell lysate
    Lane 3: A549 cell lysate
    Lane 5: 293T cell lysate
    Lane 7: MCF-7 cell lysate
    Lane 8: Mouse brain lysate
    Lane 9: Mouse liver lysate
    Lane 10: Mouse testis lysate
    Lane11: Rat liver lysate
    Lane12: Rat testis lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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