Anti-Human PELO Polyclonal Antibody (HP640014)

Name: Anti-Human PELO Polyclonal Antibody
Brand: abinScience
SKU: HP640014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HP640014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human PELO (Met1-Asp385).

Target

PELO, Protein pelota homolog

Purification

Purified by antigen affinity column.

Accession

Q9BRX2

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Protein pelota homolog is a ~43 kDa protein. Component of the Pelota-HBS1L complex, a complex that recognizes stalled ribosomes and triggers the No-Go Decay (NGD) pathway. In the Pelota-HBS1L complex, PELO recognizes ribosomes stalled at the 3' end of an mRNA and engages stalled ribosomes by destabilizing mRNA in the mRNA channel. Following mRNA extraction from stalled ribosomes by the SKI complex, the Pelota-HBS1L complex promotes recruitment of ABCE1, which drives the disassembly of stalled ribosomes, followed by degradation of damaged mRNAs as part of the NGD pathway. As part of the PINK1-regulated signaling, upon mitochondrial damage is recruited to the ribosome/mRNA-ribonucleoprotein complex associated to mitochondrial outer membrane thereby enabling the recruitment of autophagy receptors and induction of mitophagy.

1. Pisareva, VP. et al. (2011) The EMBO journal 30, 1804-17. PMID: 21448132

2. Saito, S. et al. (2013) The Journal of biological chemistry 288, 17832-43. PMID: 23667253

3. Ikeuchi, K. et al. (2016) FEBS letters 590, 3254-63. PMID: 27543824

4. Shao, S. et al. (2016) Cell 167, 1229-1240.e15. PMID: 27863242

5. Zinoviev, A. et al. (2020) Molecular cell 77, 1340-1349.e6. PMID: 32006463

6. Wu, Z. et al. (2018) Cell metabolism 28, 130-144.e7. PMID: 29861391

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PELO polyclonal antibody at 1μg/ml on various samples.
Lane 1: RAW264.7 cell lysate

References

Datasheet

Anti-Human PELO Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human PELO Polyclonal Antibody (HP640014)

Overview

Images

References

Datasheet

SDS

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