Anti-Human PGAM1 Polyclonal Antibody (HB661014)

Name: Anti-Human PGAM1 Polyclonal Antibody
Brand: abinScience
SKU: HB661014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB661014

Description

Anti-Human PGAM1 Polyclonal Antibody (HB661014) is a rabbit polyclonal antibody detecting PGAM1 in ELISA, IHC, WB. Suitable for Rattus norvegicus, Pan troglodytes, Human, Gallus gallus, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PGAM1 (Met1-Lys254).

Target

PGAM1

Purification

Purified by antigen affinity column.

Accession

P18669

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Phosphoglycerate mutase 1, 5.4.2.11, 5.4.2.4, BPG-dependent PGAM 1, Phosphoglycerate mutase isozyme B, PGAM-B, PGAM1, PGAMA, CDABP0006

Background

Phosphoglycerate mutase 1 (PGAM1) is a ~28 kDa protein. Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate, a crucial step in glycolysis, by using 2,3-bisphosphoglycerate. Also catalyzes the interconversion of (2R)-2,3-bisphosphoglycerate and (2R)-3-phospho-glyceroyl phosphate.

1. Hitosugi, T. et al. (2013) Nature communications 4, 1790. PMID: 23653202

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PAGM1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: Hela cell lysate
Lane 3: 293T cell lysate
Lane 4: Jurkat cell lysate
Lane 5: Lncap cell lysate
Lane 6: K562 cell lysate
Lane 7: U2OS cell lysate
Lane 8: 4T1 cell lysate
Lane 9: HepG2 cell lysate
Lane 10: Mouse liver lysate
Lane11: Mouse kidney lysate
Lane12: Rat liver lysate
Lane13: Rat kidney lysate

References

Datasheet

Anti-Human PGAM1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human PGAM1 Polyclonal Antibody (HB661014)

Overview

Images

References

Datasheet

SDS

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