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Anti-Human PGAM2 Polyclonal Antibody (HB660014)

Anti-Human PGAM2 Polyclonal Antibody
Anti-Human PGAM2 Polyclonal Antibody
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Overview
Catalog No.HB660014
Description
Anti-Human PGAM2 Polyclonal Antibody (HB660014) is a rabbit polyclonal antibody detecting PGAM2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PGAM2 (Met1-Lys253).
Target Phosphoglycerate mutase 2, 5.4.2.11, 5.4.2.4, BPG-dependent PGAM 2, Muscle-specific phosphoglycerate mutase, Phosphoglycerate mutase isozyme M, PGAM-M, PGAM2, PGAMM
Purification Purified by antigen affinity column.
Accession P15259
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Phosphoglycerate mutase 2 (PGAM2) is a ~28 kDa protein. Catalyzes the interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the interconversion of (2R)-2,3-bisphosphoglycerate and (2R)-3-phospho-glyceroyl phosphate, but with a reduced activity.

Note For research use only.
Images
  • Anti-Human PGAM2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PGAM2 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: A431 cell lysate
    Lane 4: Raji cell lysate
    Lane 5: U87 cell lystae
    Lane 6: Mouse kidney lysate
    Lane 7: Mouse heart lysate
    Lane 8: Mouse skeletal muscle lysate
    Lane 9: Mouse testis lysate
    Lane 10: Rat kidney lysate
    Lane11: Rat heart lysate
    Lane12: Rat skeletal muscle lysate
    Lane13: Rat testis lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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