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Anti-Human PGK2 Polyclonal Antibody (HY100014)

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Anti-Human PGK2 Polyclonal Antibody
Anti-Human PGK2 Polyclonal Antibody
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Overview
Catalog No.HY100014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PGK2 (Met1-Met417).
Target Phosphoglycerate kinase 2, PGKB, PGK2, Phosphoglycerate kinase, testis specific
Purification Purified by antigen affinity column.
Accession P07205
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Phosphoglycerate kinase 2 (PGK2) is a ~44 kDa protein. Catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate and plays a role in glycolysis and gluconeogenesis. Essential for sperm motility and male fertility. Not required for the completion of spermatogenesis.

1. Liu, XX. et al. (2016) Human reproduction (Oxford, England) 31, 273-9. PMID: 26677959
Note For research use only.
Images
  • Anti-Human PGK2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PGK2 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: U87 cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: Hela cell lysate
    Lane 4: HepG2 cell lysate
    Lane 5: A549 cell lysate
    Lane 6: Raji cell lysate
    Lane 7: Molt-4 cell lysate
    Lane 8: Mouse brain lysate
    Lane 9: Mouse heart lysate
    Lane 10: Mouse testis lysate
    Lane11: Rat brain lysate
    Lane12: Rat heart lysate
    Lane13: Rat testis lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human PGK2 Polyclonal Antibody

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