Anti-Human PHGDH Polyclonal Antibody (HT410014)

Name: Anti-Human PHGDH Polyclonal Antibody
Brand: abinScience
SKU: HT410014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HT410014

Description

Anti-Human PHGDH Polyclonal Antibody (HT410014) is a rabbit polyclonal antibody detecting PHGDH in ELISA, IHC, WB. Suitable for Bovine, Human, Macaca fascicularis, Mouse, and Pan troglodytes.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PHGDH (Met1-Phe533).

Target

2-oxoglutarate reductase, Malate dehydrogenase, 3-PGDH, PGDH3, PHGDH, D-3-phosphoglycerate dehydrogenase

Purification

Purified by antigen affinity column.

Accession

O43175

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

D-3-phosphoglycerate dehydrogenase (PHGDH) is a ~56 kDa protein. Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PHGDH polyclonal antibody at 1μg/ml on various samples.
Lane 1: HepG2 cell lysate
Lane 2: 293T cell lysate
Lane 3: MCF7 cell lysate
Lane 4: K562 cell lysate

References

Datasheet

Anti-Human PHGDH Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human PHGDH Polyclonal Antibody (HT410014)

Overview

Images

References

Datasheet

SDS

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