Anti-Human PHLPP1 Polyclonal Antibody (HT225014)

Name: Anti-Human PHLPP1 Polyclonal Antibody
Brand: abinScience
SKU: HT225014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HT225014

Description

Anti-Human PHLPP1 Polyclonal Antibody (HT225014) is a rabbit polyclonal antibody detecting PHLPP1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human PHLPP1 (Ser1168-Thr1425).

Target

KIAA0606, Pleckstrin homology domain-containing family E member 1, hSCOP, PHLPP, SCOP, PHLPP1, PH domain leucine-rich repeat-containing protein phosphatase 1, PLEKHE1, Suprachiasmatic nucleus circadian oscillatory protein, PH domain-containing family E member 1

Purification

Purified by antigen affinity column.

Accession

O60346

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

PH domain leucine-rich repeat-containing protein phosphatase 1 (PHLPP1) is a ~184 kDa protein. Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT2 and AKT3, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA. Isoform 2 seems to have a major role in regulating Akt signaling in hippocampal neurons. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and suppression of tumor growth.

1. Gao, T. et al. (2005) Molecular cell 18, 13-24. PMID: 15808505

2. Brognard, J. et al. (2007) Molecular cell 25, 917-31. PMID: 17386267

3. Gao, T. et al. (2008) The Journal of biological chemistry 283, 6300-11. PMID: 18162466

4. Qiao, M. et al. (2010) Molecular cell 38, 512-23. PMID: 20513427

5. Liu, J. et al. (2011) Molecular and cellular biology 31, 4917-27. PMID: 21986499

6. Liu, J. et al. (2009) Oncogene 28, 994-1004. PMID: 19079341

7. Li, X. et al. (2014) Gastroenterology 146, 1301-12.e1-10. PMID: 24530606

Note

For research use only.

Images

References

Datasheet

Anti-Human PHLPP1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human PHLPP1 Polyclonal Antibody (HT225014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us