Anti-Human PIEZO1 Polyclonal Antibody (HP708014)

Name: Anti-Human PIEZO1 Polyclonal Antibody
Brand: abinScience
SKU: HP708014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HP708014

Description

Anti-Human PIEZO1 Polyclonal Antibody (HP708014) is a rabbit polyclonal antibody detecting PIEZO1 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human PIEZO1 (Gln2206-Lys2426).

Target

Piezo-type mechanosensitive ion channel component 1, FAM38A, PIEZO1, Protein FAM38A, KIAA0233, Mib, Membrane protein induced by beta-amyloid treatment

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q92508, E2JF22, Q0KL00

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Piezo-type mechanosensitive ion channel component 1 (PIEZO1) is a ~286 kDa protein. Pore-forming subunit of the mechanosensitive non-specific cation Piezo channel required for rapidly adapting mechanically activated (MA) currents and has a key role in sensing touch and tactile pain. Piezo channels are homotrimeric three-blade propeller-shaped structures that utilize a cap-motion and plug-and-latch mechanism to gate their ion-conducting pathways. Generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium. Conductance to monovalent alkali ions is highest for K(+), intermediate for Na(+) and lowest for Li(+). Divalent ions except for Mn(2+) permeate the channel but more slowly than the monovalent ions and they also reduce K(+) currents.

1. Andolfo, I. et al. (2013) Blood 121, 3925-35, S1-12. PMID: 23479567

2. Albuisson, J. et al. (2013) Nature communications 4, 1884. PMID: 23695678

3. Gnanasambandam, R. et al. (2015) PloS one 10, e0125503. PMID: 25955826

4. Zhou, Z. et al. (2023) Science (New York, N.Y.) 381, 799-804. PMID: 37590348

5. McHugh, BJ. et al. (2010) Journal of cell science 123, 51-61. PMID: 20016066

6. Li, J. et al. (2014) Nature 515, 279-282. PMID: 25119035

7. Tsuchiya, M. et al. (2018) Nature communications 9, 2049. PMID: 29799007

Note

For research use only.

Images

References

Datasheet

Anti-Human PIEZO1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human PIEZO1 Polyclonal Antibody (HP708014)

Overview

Images

References

Datasheet

SDS