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Anti-Human PIH1D1 Polyclonal Antibody (HP530014)

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Overview
Catalog No.HP530014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PIH1D1 (Glu15-Ser181).
Target Nucleolar protein 17 homolog, PIH1D1, PIH1 domain-containing protein 1, NOP17
Purification Purified by antigen affinity column.
Accession Q9NWS0
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

PIH1 domain-containing protein 1 (PIH1D1) is a ~32 kDa protein. Involved in the assembly of C/D box small nucleolar ribonucleoprotein (snoRNP) particles. Recruits the SWI/SNF complex to the core promoter of rRNA genes and enhances pre-rRNA transcription. Mediates interaction of TELO2 with the R2TP complex which is necessary for the stability of MTOR and SMG1. Positively regulates the assembly and activity of the mTORC1 complex.

1. McKeegan, KS. et al. (2007) Molecular and cellular biology 27, 6782-93. PMID: 17636026
2. Zhai, N. et al. (2012) Journal of molecular cell biology 4, 231-41. PMID: 22368283
3. Kamano, Y. et al. (2013) FEBS letters 587, 3303-8. PMID: 24036451
4. Horejsí, Z. et al. (2010) Molecular cell 39, 839-50. PMID: 20864032
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-Human PIH1D1 Polyclonal Antibody

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