Anti-Human PIK3CA Polyclonal Antibody (HW719014)

Name: Anti-Human PIK3CA Polyclonal Antibody
Brand: abinScience
SKU: HW719014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HW719014

Description

Anti-Human PIK3CA Polyclonal Antibody (HW719014) is a rabbit polyclonal antibody detecting PIK3CA. Suitable for Mouse, Rat, and Human.

Highlights

●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PIK3CA (Asn345-Phe550).

Target

EC:2.7.1.137, EC:2.7.1.153, EC:2.7.11.1, PI3-kinase subunit alpha, PI3K-alpha, PI3Kalpha, PIK3CA, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphoinositide 3-kinase alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, PtdIns-3-kinase subunit alpha, PtdIns-3-kinase subunit p110-alpha, Serine/threonine protein kinase PIK3CA, p110alpha

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P42336

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PIK3CA) is a ~124 kDa protein. Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides. Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF.

1. Meier, TI. et al. (2004) Protein expression and purification 35, 218-24. PMID: 15135396

2. Buchanan, CM. et al. (2013) PloS one 8, e71337. PMID: 23936502

3. Maheshwari, S. et al. (2017) The Journal of biological chemistry 292, 13541-13550. PMID: 28676499

Note

For research use only

Images

References

Datasheet

Anti-Human PIK3CA Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human PIK3CA Polyclonal Antibody (HW719014)

Overview

Images

References

Datasheet

SDS

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