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Anti-Human PLA2G10 Polyclonal Antibody (HT102014)

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Overview
Catalog No.HT102014
Description
Anti-Human PLA2G10 Polyclonal Antibody (HT102014) is a rabbit polyclonal antibody detecting PLA2G10. Suitable for Human.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human PLA2G10 (Glu32-Asp165).
Target EC:3.1.1.4, GX sPLA2, Group 10 secretory phospholipase A2, Group X secretory phospholipase A2, PLA2G10, Phosphatidylcholine 2-acylhydrolase 10, sPLA2-X
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O15496
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Group 10 secretory phospholipase A2 (PLA2G10) is a ~18 kDa protein. Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids with preference for phosphatidylcholines and phosphatidylglycerols over phosphatidylethanolamines. Preferentially releases sn-2 omega-6 and omega-3 polyunsaturated fatty acyl (PUFA) chains over saturated fatty acyls. Contributes to phospholipid remodeling of very low-density lipoprotein (VLDL), low-density lipoprotein (LDL) and high-density lipoprotein (HDL) particles. Hydrolyzes LDL phospholipids releasing unsaturated fatty acids that regulate macrophage differentiation toward foam cells.

1. Hanasaki, K. et al. (2002) The Journal of biological chemistry 277, 29116-24. PMID: 12021277
2. Cupillard, L. et al. (1997) The Journal of biological chemistry 272, 15745-52. PMID: 9188469
3. Singer, AG. et al. (2002) The Journal of biological chemistry 277, 48535-49. PMID: 12359733
4. Gora, S. et al. (2006) Biochimica et biophysica acta 1761, 1093-9. PMID: 16962371
5. Koduri, RS. et al. (2002) The Journal of biological chemistry 277, 5849-57. PMID: 11694541
6. Mitsuishi, M. et al. (2006) The Biochemical journal 393, 97-106. PMID: 16146426
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human PLA2G10 Polyclonal Antibody

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