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Anti-Human PLA2G5 Polyclonal Antibody (HA627014)

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Overview
Catalog No.HA627014
Description
Anti-Human PLA2G5 Polyclonal Antibody (HA627014) is a rabbit polyclonal antibody detecting Phospholipase A2 group V; 3.1.1.4; PLA2-10; Phosphatidylcholine 2-acylhydrolase 5; PLA2G5 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant human PLA2G5 (Gly21-Ser138).
Target Phospholipase A2 group V; 3.1.1.4; PLA2-10; Phosphatidylcholine 2-acylhydrolase 5; PLA2G5
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P39877
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Phospholipase A2 group V (PLA2G5) is a ~15 kDa protein. Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity), preferentially releasing fatty acyl groups with a low degree of unsaturation such as oleoyl (C18:1) and linoleoyl (C18:2) groups. Hydrolyzes low-density lipoprotein (LDL) phospholipids releasing unsaturated fatty acids that drive macrophage polarization toward an M2 phenotype. May act in an autocrine and paracrine manner. Contributes to lipid remodeling of cellular membranes at different subcellular locations and generation of lipid mediators involved in pathogen clearance.

1. Chen, J. et al. (1994) The Journal of biological chemistry 269, 2365-8. PMID: 8300559
2. Sun, YX. et al. (2004) The Biochemical journal 380, 749-56. PMID: 14998370
3. Hsu, YH. et al. (2013) PloS one 8, e59267. PMID: 23533611
4. Rubio, JM. et al. (2015) Journal of immunology (Baltimore, Md. : 1950) 194, 3327-39. PMID: 25725101
5. Koduri, RS. et al. (2002) The Journal of biological chemistry 277, 5849-57. PMID: 11694541
6. Kim, YJ. et al. (2002) The Journal of biological chemistry 277, 36479-88. PMID: 12124392
7. Muñoz, NM. et al. (2003) The Journal of biological chemistry 278, 38813-20. PMID: 12796497
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human PLA2G5 Polyclonal Antibody

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