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Anti-Human PLK3 Polyclonal Antibody (HA090014)

Anti-Human PLK3 Polyclonal Antibody
Anti-Human PLK3 Polyclonal Antibody
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Overview
Catalog No.HA090014
Description
Anti-Human PLK3 Polyclonal Antibody (HA090014) is a rabbit polyclonal antibody detecting PLK3 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PLK3 (Thr426-Ala646).
Target FGF-inducible kinase, FNK, PLK-3, Serine/threonine-protein kinase PLK3, Cytokine-inducible serine/threonine-protein kinase, PLK3, PRK, CNK, Polo-like kinase 3, Proliferation-related kinase
Purification Purified by antigen affinity column.
Accession Q9H4B4
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Serine/threonine-protein kinase PLK3 is a ~71 kDa protein. Serine/threonine-protein kinase involved in cell cycle regulation, response to stress and Golgi disassembly. Polo-like kinases act by binding and phosphorylating proteins that are already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/TP53, p73/TP73, PTEN, TOP2A and VRK1. Involved in cell cycle regulation: required for entry into S phase and cytokinesis. Phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis.

Note For research use only.
Images
  • Anti-Human PLK3 Polyclonal Antibody

    Western blot

    Lane 1: Hela cell lysate
    Lane 2: H1299 cell lysate
    Lane 3: K562 cell lysate
    Lane 4: 293T cell lysate
    Lane 5: A549 cell lysate
    Lane 6: SKOV3 cell lysate
    Lane 7: Mouse brain lysate
    Lane 8: Mouse spleen lysate
    Lane 9: Rat brain lysate
    Lane 10: Rat heart lysate
    Lane11: Rat spleen lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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