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Anti-Human PMFBP1 Polyclonal Antibody (HP088014)

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Overview
Catalog No.HP088014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human PMFBP1 (Met1-Asn150).
Target Polyamine-modulated factor 1-binding protein 1, PMFBP1, PMF-1-binding protein
Purification Purified by antigen affinity column.
Accession Q8TBY8
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Polyamine-modulated factor 1-binding protein 1 (PMFBP1) is a ~117 kDa protein. Required for normal spermatogenesis. It functions as a scaffold protein that attaches the sperm head-tail connecting piece to the nuclear envelope, thus maintaining sperm head and tail integrity. May also be involved in the general organization of cellular cytoskeleton.

1. Haidl, G. et al. (1991) Human reproduction (Oxford, England) 6, 1431-8. PMID: 1770140
2. Zhu, F. et al. (2018) American journal of human genetics 103, 188-199. PMID: 30032984
3. Sha, YW. et al. (2019) Clinical genetics 95, 277-286. PMID: 30298696
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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