Anti-Human PNPLA3 Polyclonal Antibody (HA111014)

Name: Anti-Human PNPLA3 Polyclonal Antibody
Brand: abinScience
SKU: HA111014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HA111014

Description

Anti-Human PNPLA3 Polyclonal Antibody (HA111014) is a rabbit polyclonal antibody detecting PNPLA3 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PNPLA3 (Met1-Lys252).

Target

1-acylglycerol-3-phosphate O-acyltransferase PNPLA3, iPLA2-epsilon, C22orf20, Lysophosphatidic acid acyltransferase, Acylglycerol transacylase, Calcium-independent phospholipase A2-epsilon, Patatin-like phospholipase domain-containing protein 3, ADPN, Adiponutrin, PNPLA3

Purification

Purified by antigen affinity column.

Accession

Q9NST1

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

1-acylglycerol-3-phosphate O-acyltransferase PNPLA3 is a ~52 kDa protein. Specifically catalyzes coenzyme A (CoA)-dependent acylation of 1-acyl-sn-glycerol 3-phosphate (2-lysophosphatidic acid/LPA) to generate phosphatidic acid (PA), an important metabolic intermediate and precursor for both triglycerides and glycerophospholipids. Does not esterify other lysophospholipids. Acyl donors are long chain (at least C16) fatty acyl-CoAs: arachidonoyl-CoA, linoleoyl-CoA, oleoyl-CoA and at a lesser extent palmitoyl-CoA. Additionally possesses low triacylglycerol lipase and CoA-independent acylglycerol transacylase activities and thus may play a role in acyl-chain remodeling of triglycerides. In vitro may express hydrolytic activity against glycerolipids triacylglycerol, diacylglycerol and monoacylglycerol, with a strong preference for oleic acid as the acyl moiety.

1. Kumari, M. et al. (2012) Cell metabolism 15, 691-702. PMID: 22560221

2. Jenkins, CM. et al. (2004) The Journal of biological chemistry 279, 48968-75. PMID: 15364929

3. He, S. et al. (2010) The Journal of biological chemistry 285, 6706-15. PMID: 20034933

4. Huang, Y. et al. (2011) The Journal of biological chemistry 286, 37085-93. PMID: 21878620

Note

For research use only.

Images

References

Datasheet

Anti-Human PNPLA3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human PNPLA3 Polyclonal Antibody (HA111014)

Overview

Images

References

Datasheet

SDS

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