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Anti-Human PNPLA4 Polyclonal Antibody (HA636014)

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Overview
Catalog No.HA636014
Description
Anti-Human PNPLA4 Polyclonal Antibody (HA636014) is a rabbit polyclonal antibody detecting Patatin-like phospholipase domain-containing protein 4; 3.1.1.3; Calcium-independent phospholipase A2-eta; iPLA2-eta; 3.1.1.4; Protein GS2; PNPLA4; DXS1283E; GS2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human PNPLA4 (Met1-Glu253).
Target Patatin-like phospholipase domain-containing protein 4; 3.1.1.3; Calcium-independent phospholipase A2-eta; iPLA2-eta; 3.1.1.4; Protein GS2; PNPLA4; DXS1283E; GS2
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P41247
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Patatin-like phospholipase domain-containing protein 4 (PNPLA4) is a ~27 kDa protein. Has abundant triacylglycerol lipase activity. Transfers fatty acid from triglyceride to retinol, hydrolyzes retinylesters, and generates 1,3-diacylglycerol from triglycerides. Additionally possesses acylglycerol transacylase and phospholipase A2 activities.

1. Jenkins, CM. et al. (2004) The Journal of biological chemistry 279, 48968-75. PMID: 15364929
2. Lake, AC. et al. (2005) Journal of lipid research 46, 2477-87. PMID: 16150821
3. Gao, JG. et al. (2007) Biochemical and biophysical research communications 360, 501-6. PMID: 17603008
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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