Anti-Human POLI Polyclonal Antibody (HP553014)

Name: Anti-Human POLI Polyclonal Antibody
Brand: abinScience
SKU: HP553014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HP553014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human POLI (Arg53-Lys439).

Target

RAD30 homolog B, RAD30B, DNA polymerase iota, Eta2, POLI

Purification

Purified by antigen affinity column.

Accession

Q9UNA4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA polymerase iota (POLI) is a ~83 kDa protein. Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine.

1. Tissier, A. et al. (2000) The EMBO journal 19, 5259-66. PMID: 11013228

2. Frank, EG. et al. (2001) The EMBO journal 20, 2914-22. PMID: 11387224

3. Haracska, L. et al. (2003) Genes & development 17, 2777-85. PMID: 14630940

4. Washington, MT. et al. (2004) Molecular and cellular biology 24, 5687-93. PMID: 15199127

5. Zhou, Y. et al. (2010) Nucleic acids research 38, 5036-46. PMID: 20388628

6. Nair, DT. et al. (2004) Nature 430, 377-80. PMID: 15254543

Note

For research use only.

Images

References

Datasheet

Anti-Human POLI Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human POLI Polyclonal Antibody (HP553014)

Overview

Images

References

Datasheet

SDS

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