Anti-Human POLR3D Polyclonal Antibody (HY534014)

Name: Anti-Human POLR3D Polyclonal Antibody
Brand: abinScience
SKU: HY534014
Price: 168 USD
Availability: InStock

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Overview
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SDS

Overview

Catalog No.

HY534014

Description

Anti-Human POLR3D Polyclonal Antibody (HY534014) is a rabbit polyclonal antibody detecting POLR3D in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human POLR3D (Val249-Arg398).

Target

DNA-directed RNA polymerase III subunit RPC4, RNA polymerase III subunit C4, DNA-directed RNA polymerase III subunit D, Protein BN51, RNA polymerase III 47 kDa subunit, RPC53 homolog, POLR3D, BN51, BN51T

Purification

Purified by antigen affinity column.

Accession

P05423

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA-directed RNA polymerase III subunit RPC4 (POLR3D) is a ~44 kDa protein. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Assembles with POLR3E/RPC5 forming a subcomplex that binds the Pol III core. Enables recruitment of Pol III at transcription initiation site and drives transcription initiation from both type 2 and type 3 DNA promoters. Required for efficient transcription termination and reinitiation.

1. Hu, P. et al. (2002) Molecular and cellular biology 22, 8044-55. PMID: 12391170

2. Canella, D. et al. (2010) Genome research 20, 710-21. PMID: 20413673

3. Girbig, M. et al. (2021) Nature structural & molecular biology 28, 210-219. PMID: 33558764

4. Hou, H. et al. (2021) Nature communications 12, 6135. PMID: 34675218

5. Van Bortle, K. et al. (2022) Nature communications 13, 3007. PMID: 35637192

6. Ablasser, A. et al. (2009) Nature immunology 10, 1065-72. PMID: 19609254

7. Chiu, YH. et al. (2009) Cell 138, 576-91. PMID: 19631370

Note

For research use only.

Images

References

Datasheet

Anti-Human POLR3D Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-Human POLR3D Polyclonal Antibody (HY534014)

Overview

Images

References

Datasheet

SDS

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