Anti-Human PPM1D Polyclonal Antibody (HT072014)

Name: Anti-Human PPM1D Polyclonal Antibody
Brand: abinScience
SKU: HT072014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HT072014

Description

Anti-Human PPM1D Polyclonal Antibody (HT072014) is a rabbit polyclonal antibody detecting PPM1D in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PPM1D (Ser96-Leu395).

Target

Protein phosphatase 1D, 3.1.3.16, Protein phosphatase 2C isoform delta, PP2C-delta, Protein phosphatase magnesium-dependent 1 delta, p53-induced protein phosphatase 1, PPM1D, WIP1

Purification

Purified by antigen affinity column.

Accession

O15297

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Protein phosphatase 1D (PPM1D) is a ~66 kDa protein. Involved in the negative regulation of p53 expression. Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. Mediates MAPK14 dephosphorylation and inactivation. Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity.

1. Ruark, E. et al. (2013) Nature 493, 406-10. PMID: 23242139

2. Lu, X. et al. (2005) Genes & development 19, 1162-74. PMID: 15870257

3. Fujimoto, H. et al. (2006) Cell death and differentiation 13, 1170-80. PMID: 16311512

4. An, H. et al. (2011) PloS one 6, e16427. PMID: 21283629

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human PPM1D polyclonal antibody at 1ug/mL on recombinant Human PPM1D (Catalog No. HT072012).

References

Datasheet

Anti-Human PPM1D Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human PPM1D Polyclonal Antibody (HT072014)

Overview

Images

References

Datasheet

SDS

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