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Anti-Human PPP1CB Polyclonal Antibody (HX142014)

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Overview
Catalog No.HX142014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PPP1CB (Met1-Arg327).
Target Serine/threonine-protein phosphatase PP1-beta catalytic subunit, PPP1CD, PP-1B, PPP1CB
Purification Purified by antigen affinity column.
Accession P62140
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Serine/threonine-protein phosphatase PP1-beta catalytic subunit (PPP1CB) is a ~37 kDa protein. Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation.

1. Nie, H. et al. (2013) Nature medicine 19, 322-8. PMID: 23396208
2. Liau, NPD. et al. (2022) Nature 609, 400-407. PMID: 35768504
3. Kwon, JJ. et al. (2022) Nature 609, 408-415. PMID: 35831509
4. Bonsor, DA. et al. (2022) Nature structural & molecular biology 29, 966-977. PMID: 36175670
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human PPP1CB Polyclonal Antibody

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