Anti-Human PPP2CA Polyclonal Antibody (HX136014)

Name: Anti-Human PPP2CA Polyclonal Antibody
Brand: abinScience
SKU: HX136014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HX136014

Description

Anti-Human PPP2CA Polyclonal Antibody (HX136014) is a rabbit polyclonal antibody detecting PPP2CA in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Chicken.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PPP2CA (Met1-Leu309).

Target

Replication protein C, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, RP-C, PP2A-alpha, PPP2CA

Purification

Purified by antigen affinity column.

Accession

P67775

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform (PPP2CA) is a ~35 kDa protein. Catalytic subunit of protein phosphatase 2A (PP2A), a serine/threonine phosphatase involved in the regulation of a wide variety of enzymes, signal transduction pathways, and cellular events. PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Can dephosphorylate various proteins, such as SV40 large T antigen, AXIN1, p53/TP53, PIM3, WEE1.

1. Abraham, D. et al. (2000) The Journal of biological chemistry 275, 22300-4. PMID: 10801873

2. Losman, JA. et al. (2003) The Journal of biological chemistry 278, 4800-5. PMID: 12473674

3. Li, HH. et al. (2007) The EMBO journal 26, 402-11. PMID: 17245430

4. Watkins, GR. et al. (2012) The Journal of biological chemistry 287, 24207-15. PMID: 22613722

5. Zheng, H. et al. (2020) Science (New York, N.Y.) 370. PMID: 33243860

6. Vervoort, SJ. et al. (2021) Cell 184, 3143-3162.e32. PMID: 34004147

7. Hsu, W. et al. (1999) The Journal of biological chemistry 274, 3439-45. PMID: 9920888

8. Li, F. et al. (2020) Molecular cell 80, 410-422.e6. PMID: 33108758

10. Becher, J. et al. (2018) Developmental cell 47, 592-607.e6. PMID: 30513302

Note

For research use only.

Images

References

Datasheet

Anti-Human PPP2CA Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human PPP2CA Polyclonal Antibody (HX136014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us