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Anti-Human PRAP1 Polyclonal Antibody (HA024014)

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Overview
Catalog No.HA024014
Description
Anti-Human PRAP1 Polyclonal Antibody (HA024014) is a rabbit polyclonal antibody detecting Proline-rich acidic protein 1; Epididymis tissue protein Li 178; Uterine-specific proline-rich acidic protein; PRAP1; UPA; UNQ608/PRO1195 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant human PRAP1 (Val21-Gln151).
Target Proline-rich acidic protein 1; Epididymis tissue protein Li 178; Uterine-specific proline-rich acidic protein; PRAP1; UPA; UNQ608/PRO1195
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q96NZ9
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Proline-rich acidic protein 1 (PRAP1) is a ~17 kDa protein. Lipid-binding protein which promotes lipid absorption by facilitating MTTP-mediated lipid transfer (mainly triglycerides and phospholipids) and MTTP-mediated apoB lipoprotein assembly and secretion. Protects the gastrointestinal epithelium from irradiation-induced apoptosis. May play an important role in maintaining normal growth homeostasis in epithelial cells. Involved in p53/TP53-dependent cell survival after DNA damage. May down-regulate the expression of MAD1L1 and exert a suppressive role in mitotic spindle assembly checkpoint in hepatocellular carcinomas.

1. Zhang, J. et al. (2003) Cancer research 63, 6658-65. PMID: 14583459
2. Huang, BH. et al. (2012) Cell death & disease 3, e442. PMID: 23235459
3. Sze, KM. et al. (2014) The Journal of pathology 233, 51-60. PMID: 24374861
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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