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Anti-Human PRPF6 Polyclonal Antibody (HD153014)

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Anti-Human PRPF6 Polyclonal Antibody
Anti-Human PRPF6 Polyclonal Antibody
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Overview
Catalog No.HD153014
Description
Anti-Human PRPF6 Polyclonal Antibody (HD153014) is a rabbit polyclonal antibody detecting PRPF6. Suitable for Rat, Human, and Mouse.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human PRPF6 (Lys606-Phe941).
Target ANT-1, Androgen receptor N-terminal domain-transactivating protein 1, C20orf14, PRP6 homolog, PRPF6, Pre-mRNA-processing factor 6, U5 snRNP-associated 102 kDa protein, U5-102 kDa protein
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession O94906
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Pre-mRNA-processing factor 6 (PRPF6) is a ~106 kDa protein. Involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, one of the building blocks of the spliceosome. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation.

1. Schneider, M. et al. (2010) Nature structural & molecular biology 17, 216-21. PMID: 20118938
2. Tanackovic, G. et al. (2011) American journal of human genetics 88, 643-9. PMID: 21549338
3. Bertram, K. et al. (2017) Cell 170, 701-713.e11. PMID: 28781166
Note For research use only
Images
  • Anti-Human PRPF6 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PRPF6 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: K562 cell lysate
    Lane 2: HL-60 cell lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human PRPF6 Polyclonal Antibody

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