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Anti-Human PSMA4 Polyclonal Antibody (HB983014)

Anti-Human PSMA4 Polyclonal Antibody
Anti-Human PSMA4 Polyclonal Antibody
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Overview
Catalog No.HB983014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PSMA4 (Met1-Lys261).
Target Proteasome component C9, Macropain subunit C9, Multicatalytic endopeptidase complex subunit C9, Proteasome subunit L, PSC9, HC9, Proteasome subunit alpha type-4, PSMA4
Purification Purified by antigen affinity column.
Accession P25789
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Proteasome subunit alpha type-4 (PSMA4) is a ~29 kDa protein. Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation.

Note For research use only.
Images
  • Anti-Human PSMA4 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PSMA4 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: BxPC-3 cell lysate
    Lane 2: K562 cell lysate
    Lane 3: U937 cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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