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Anti-Human PSMA7 Polyclonal Antibody (HT374014)

Anti-Human PSMA7 Polyclonal Antibody
Anti-Human PSMA7 Polyclonal Antibody
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Overview
Catalog No.HT374014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PSMA7 (His15-Ser248).
Target Proteasome subunit RC6-1, HSPC, Proteasome subunit XAPC7, Proteasome subunit alpha type-7, PSMA7
Purification Purified by antigen affinity column.
Accession O14818
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Proteasome subunit alpha type-7 (PSMA7) is a ~27 kDa protein. Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation.

Note For research use only.
Images
  • Anti-Human PSMA7 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-PSMA7 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: HepG2 cell lysate
    Lane 2: Lncap cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: Mouse liver lysate
    Lane 5: Mouse lung lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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