Anti-Human PSMB5 Polyclonal Antibody (HB987014)

Name: Anti-Human PSMB5 Polyclonal Antibody
Brand: abinScience
SKU: HB987014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HB987014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PSMB5 (Gly59-Pro263).

Target

MB1, PSMB5, Proteasome subunit MB1, X, Macropain epsilon chain, Proteasome subunit beta type-5, Multicatalytic endopeptidase complex epsilon chain, LMPX, Proteasome subunit X, Proteasome chain 6, Proteasome epsilon chain

Purification

Purified by antigen affinity column.

Accession

P28074

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Proteasome subunit beta type-5 (PSMB5) is a ~28 kDa protein. Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PSMB5 polyclonal antibody at 1μg/ml on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: Hela cell lysate
Lane 3: HepG2 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: LO2 cell lysate
Lane 6: U2OS cell lysate
Lane 7: K562 cell lysate
Lane 8: PC-3 cell lysate
Lane 9: MCF-7 cell lysate

References

Datasheet

Anti-Human PSMB5 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human PSMB5 Polyclonal Antibody (HB987014)

Overview

Images

References

Datasheet

SDS

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