Anti-Human PSMD14 Polyclonal Antibody (HT284014)

Name: Anti-Human PSMD14 Polyclonal Antibody
Brand: abinScience
SKU: HT284014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HT284014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human PSMD14 (Gln30-Lys209).

Target

26S proteasome non-ATPase regulatory subunit 14, 26S proteasome-associated PAD1 homolog 1, POH1, 26S proteasome regulatory subunit RPN11, PSMD14

Purification

Purified by antigen affinity column.

Accession

O00487

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ubiquitin C-terminal hydrolase PSMD14 is a ~34 kDa protein. Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. The PSMD14 subunit is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains within the complex. Plays a role in response to double-strand breaks (DSBs): acts as a regulator of non-homologous end joining (NHEJ) by cleaving 'Lys-63'-linked polyubiquitin, thereby promoting retention of JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation.

1. Spataro, V. et al. (1997) The Journal of biological chemistry 272, 30470-5. PMID: 9374539

2. Kanayama, HO. et al. (1992) European journal of biochemistry 206, 567-78. PMID: 1317798

3. Butler, LR. et al. (2012) The EMBO journal 31, 3918-34. PMID: 22909820

4. Bustamante, HA. et al. (2020) Cells 9. PMID: 32210007

Note

For research use only.

Images

References

Datasheet

Anti-Human PSMD14 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human PSMD14 Polyclonal Antibody (HT284014)

Overview

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References

Datasheet

SDS

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