Anti-Human PSME3 Polyclonal Antibody (HX952014)

Name: Anti-Human PSME3 Polyclonal Antibody
Brand: abinScience
SKU: HX952014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HX952014

Description

Anti-Human PSME3 Polyclonal Antibody (HX952014) is a rabbit polyclonal antibody detecting PSME3 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human PSME3 (Ser111-Tyr254).

Target

Activator of multicatalytic protease subunit 3, Proteasome activator complex subunit 3, PA28g, 11S regulator complex subunit gamma, PA28gamma, Ki nuclear autoantigen, REG-gamma, PSME3, Proteasome activator 28 subunit gamma

Purification

Purified by antigen affinity column.

Accession

P61289

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Proteasome activator complex subunit 3 (PSME3) is a ~29 kDa protein. Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition.

1. Magni, M. et al. (2014) Nucleic acids research 42, 13150-60. PMID: 25361978

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PSME3 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: Hela cell lysate
Lane 3: K562 cell lysate
Lane 4: 293T cell lysate

References

Datasheet

Anti-Human PSME3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human PSME3 Polyclonal Antibody (HX952014)

Overview

Images

References

Datasheet

SDS

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