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Anti-Human PTGES3/P23 Polyclonal Antibody (HS799014)

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Anti-Human PTGES3/P23 Polyclonal Antibody
Anti-Human PTGES3/P23 Polyclonal Antibody
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Overview
Catalog No.HS799014
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PTGES3/P23 (Met1-Glu160).
Target PTGES3, Hsp90 co-chaperone, P23, Progesterone receptor complex p23, Prostaglandin E synthase 3, cPGES, Telomerase-binding protein p23, Cytosolic prostaglandin E2 synthase, TEBP
Purification Purified by antigen affinity column.
Accession Q15185
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Prostaglandin E synthase 3 (PTGES3/P23) is a ~18 kDa protein. Cytosolic prostaglandin synthase that catalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes. Facilitates HIF alpha proteins hydroxylation via interaction with EGLN1/PHD2, leading to recruit EGLN1/PHD2 to the HSP90 pathway.

1. Tanioka, T. et al. (2000) The Journal of biological chemistry 275, 32775-82. PMID: 10922363
2. Forsythe, HL. et al. (2001) The Journal of biological chemistry 276, 15571-4. PMID: 11274138
3. Freeman, BC. et al. (2002) Science (New York, N.Y.) 296, 2232-5. PMID: 12077419
4. Song, D. et al. (2014) The Journal of biological chemistry 289, 14656-65. PMID: 24711448
Note For research use only.
Images
  • Anti-Human PTGES3/P23 Polyclonal Antibody

    Western blot

    Lane 1: A549 cell lysate
    Lane 2: PC-3 cell lysate
    Lane 3: Hela cell lysate
    Lane 4: HepG2 cell lysate
    Lane 5: NIH3T3 cell lysate
    Lane 6: 293T cell lysate
    Lane 7: Mouse testis lysate
    Lane 8: Mouse brain lysate
    Lane 9: Mouse spleen lysate
    Lane 10: Rat testis lysate
    Lane11: Rat brain lysate

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human PTGES3/P23 Polyclonal Antibody

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