Anti-Human PTTG1 Polyclonal Antibody (HF630014)

Name: Anti-Human PTTG1 Polyclonal Antibody
Brand: abinScience
SKU: HF630014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HF630014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PTTG1 (Asn121-Asp190).

Target

PTTG1, Esp1-associated protein, EAP1, Securin, TUTR1, PTTG, hPTTG, Pituitary tumor-transforming gene 1 protein, Tumor-transforming protein 1

Purification

Purified by antigen affinity column.

Accession

O95997

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Securin (PTTG1) is a ~22 kDa protein. Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PTTG1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Daudi cell lysate

References

Datasheet

Anti-Human PTTG1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human PTTG1 Polyclonal Antibody (HF630014)

Overview

Images

References

Datasheet

SDS

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