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Anti-Human PYCR1 Polyclonal Antibody (HW791014)

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Overview
Catalog No.HW791014
Description
Anti-Human PYCR1 Polyclonal Antibody (HW791014) is a rabbit polyclonal antibody detecting PYCR1 in ELISA, IHC, WB. Suitable for Bovine, Mus musculus, and Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman, Mouse
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human PYCR1 (Met1-Asp319).
Target PYCR1
Purification Purified by antigen affinity column.
Accession P32322
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesPyrroline-5-carboxylate reductase 1, mitochondrial, P5C reductase 1, P5CR 1, 1.5.1.2, PYCR1
Background

Pyrroline-5-carboxylate reductase 1, mitochondrial (PYCR1) is a ~33 kDa protein. Oxidoreductase that catalyzes the last step in proline biosynthesis, which corresponds to the reduction of pyrroline-5-carboxylate to L-proline using NAD(P)H. At physiologic concentrations, has higher specific activity in the presence of NADH. Involved in the cellular response to oxidative stress.

1. Meng, Z. et al. (2006) Journal of molecular biology 359, 1364-77. PMID: 16730026
2. Reversade, B. et al. (2009) Nature genetics 41, 1016-21. PMID: 19648921
3. De Ingeniis, J. et al. (2012) PloS one 7, e45190. PMID: 23024808
4. Christensen, EM. et al. (2017) The Journal of biological chemistry 292, 7233-7243. PMID: 28258219
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human PYCR1 Polyclonal Antibody

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